RID讀取器是一種經(jīng)濟(jì)高效且緊湊的儀器，旨在簡化和加速讀取沉淀素徑向**擴(kuò)散環(huán)的過程。
這款便攜式儀器針對使用RID板的所有規(guī)模的實(shí)驗(yàn)室，通過*少的用戶輸入就可以有效地定量已知蛋白質(zhì)的濃度，從而減少了用戶錯(cuò)誤。
RID閱讀器具有一個(gè)內(nèi)置攝像機(jī)，可以將沉淀環(huán)的大而清晰的圖像傳輸?shù)接?jì)算機(jī)屏幕上，以提高測量精度。
直觀的軟件使用戶可以從多個(gè)選項(xiàng)中進(jìn)行選擇，以根據(jù)自動(dòng)生成的校準(zhǔn)曲線計(jì)算結(jié)果，從而提高對結(jié)果的信心。
可以保存數(shù)據(jù)，校準(zhǔn)曲線和圖像以備將來參考，從而為培訓(xùn)和質(zhì)量控制提供了有用的工具。
該技術(shù)基于抗原和抗體的復(fù)合以產(chǎn)生可見的沉淀素環(huán)。沉淀素環(huán)的直徑與樣品中蛋白質(zhì)的含量成正比，可以使用目鏡進(jìn)行測量，或者使用Binding Sites Digital RID Plate Reader進(jìn)行更**的測量。
我們的大多數(shù)RID分析均基于常規(guī)抗體-抗原技術(shù)。在需要提高靈敏度的地方，我們開發(fā)了NANORID ?技術(shù)。這些試劑盒使用乳膠增強(qiáng)抗體，即使在低蛋白質(zhì)濃度下也能進(jìn)行準(zhǔn)確定量。
套件功能
多種方法：我們所有的RID分析均包含執(zhí)行三種不同分析方法的程序：
RID參考表（Mancini）
完全擴(kuò)散（曼奇尼）
不完全擴(kuò)散（Fahey和McKelvey）
具有成本效益： RID板可以分段以允許每個(gè)板使用不止一次
更高的精度和精密度：提供了樣品稀釋劑以減少樣品之間的粘度差異
帶有視頻攝像頭技術(shù)的放大和清晰定義的戒指顯示在計(jì)算機(jī)屏幕上
輕松定量環(huán)尺寸可提高結(jié)果準(zhǔn)確性
*少的維護(hù)和快速的校準(zhǔn)，再加上簡單的軟件，使得數(shù)字RID讀板機(jī)非常人性化
明亮的內(nèi)部光源
實(shí)現(xiàn)一致且恒定的查看以進(jìn)行**測量
RID板保持在閱讀器中
與更傳統(tǒng)的閱讀方法相比，穩(wěn)定觀看戒指可減少用戶出錯(cuò)的機(jī)會(huì)
校準(zhǔn)曲線由軟件繪制并自動(dòng)計(jì)算結(jié)果
提高實(shí)驗(yàn)室效率并消除轉(zhuǎn)錄錯(cuò)誤的機(jī)會(huì)
所有數(shù)據(jù)和圖像均可存儲(chǔ)
無需笨重的紙質(zhì)文件，并且可以快速/輕松地訪問所有數(shù)據(jù)
數(shù)字RID技術(shù)規(guī)范
LED陣列光源
尺寸：220mm（W）140mm（H）160mm（D）; 8.66英寸（寬）x 5.51英寸（高）x 6.30英寸（深）
儀器重量：1.14 kg；2.51磅
CCR視頻系統(tǒng)：628 x 582像素
計(jì)算機(jī)要求：USB端口時(shí)，Windows ® XP 2000，XP，Vista和Windows ® 7
軟件
徑向**擴(kuò)散方法各不相同，并且可以幾種不同方式計(jì)算結(jié)果。RIDRead軟件包的靈活性允許用戶從校準(zhǔn)曲線中選擇其**的結(jié)果計(jì)算方法：
如果精度至關(guān)重要，則使用線性曲線
如果需要快速求出結(jié)果，請選擇二項(xiàng)式曲線（使用Fahey方法）
點(diǎn)對點(diǎn)曲線用于功能補(bǔ)體測定
The RID Reader is a cost effective & compact instrument, designed to simplify & speed-up the process of reading precipitin radial immunodiffusion rings.
This portable instrument is aimed at all sizes of laboratories using RID plates, decreasing user error by efficiently quantifying the concentrations of known proteins with minimal user input.
The RID Reader has a built-in video camera that transmits large, clear images of the precipitin rings to the computer screen to improve measurement precision.
The intuitive software enables the user to select from several options for the calculation of results from an automatically generated calibration curve to increase confidence in results.
Data, calibration curves and images can be saved for future reference, providing a useful tool for training and quality control.
The technique is based on the complexing of antigen and antibody to produce a visible precipitin ring. The diameter of the precipitin ring is directly proportional to the amount of protein present in the sample and can be measured using an eye piece or, for more precise measurement, with Binding Sites Digital RID Plate Reader.
The majority of our RID assays are based on conventional antibody-antigen technology. Where increased sensitivity is required, we have developed NANORID? technology. These kits use latex enhanced antibodies to give accurate quantitation, even at low concentrations of protein.
Kit Features
Multiple Methods: All of our RID assays contain the procedures for performing three different assay methods:
RID reference table (Mancini)
Complete diffusion (Mancini)
Incomplete diffusion (Fahey and McKelvey)
Cost efficient: RID plates can be sectioned to allow each plate to be used more than once
Improved Accuracy and Precision: A sample diluent is provided to reduce viscosity differences between samples
Enlarged & clearly defined rings are displayed on the computer screen with video camera technology
Easy quantification of ring size increases accuracy of results
Minimal maintenance and quick calibration combined with simple software make the digital RID plate reader very user friendly
Bright internal light source
Enables consistent & constant viewing for precise measurement
RID plate is kept still in the reader
Steady viewing of the rings decreases chances of user error compared to more traditional reading methods
Calibration curves are drawn by the software & results are calculated automatically
Increases lab efficiency & negates the chance of transcription errors
All data & images can be stored
No need for bulky paper files & all data can be quickly / easily accessed
Digital RID technical specifications
LED array light source
Dimensions: 220mm (W) 140mm (H) 160mm (D); 8.66in (W) x 5.51in (H) x 6.30in (D)
Instrument Weight: 1.14 kg; 2.51 lbs
CCR video system: 628 x 582 pixels
Computer Requirements: USB Port, Windows® XP 2000, XP, Vista & Windows® 7
Software
Radial immunodiffusion methods vary and results can be calculated in several different ways. The flexibility of the RIDRead package allows the user to select their preferred option for result calculation from a calibration curve:
A linear curve is used if accuracy is of primary importance
Choose a binomial curve if results are required quickly (uses a Fahey method)
A point-to-point curve is used for functional complement assays