CyToxiLux? , GranToxiLux?, and PanToxiLux? Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte Principle of assays: Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence mi
CyToxiLux® , GranToxiLux®, and PanToxiLux?
Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits
A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte
Principle of assays:
Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence microscopy. In each kit cleavage of a cell permeable fluorogenic substrate reports the following activities:
CyToxiLux® : downstream caspase
GranToxiLux®: granzyme B
PanToxiLux?: granzyme B and upstream caspase
Measurements with CyToxiLux®, GranToxiLux®, and PanToxiLuxTM are superior to bulk assays e.g., LDH and 51Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity (relatively weak CTL responses against subdominant epitopes are detectable).
Detailed CyToxiLux® PLUS! instructions are available for download in PDF format
Detailed GranToxiLux® PLUS! instructions are available for download in PDF format
Detailed PanToxiLux® instructions are available for download in PDF format
Detailed PanToxiLux? P2D2 instructions are available for download in PDF format
CyToxiLux® , GranToxiLux®, and PanToxiLux?
Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits
A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte |
Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence microscopy. In each kit cleavage of a cell permeable fluorogenic substrate reports the following activities:
CyToxiLux® : downstream caspase |
GranToxiLux®: granzyme B |
PanToxiLux?: granzyme B and upstream caspase |
Measurements with CyToxiLux®, GranToxiLux®, and PanToxiLuxTM are superior to bulk assays e.g., LDH and 51Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity (relatively weak CTL responses against subdominant epitopes are detectable).
Detailed CyToxiLux® PLUS! instructions are available for download in PDF format
Detailed GranToxiLux® PLUS! instructions are available for download in PDF format
Detailed PanToxiLux® instructions are available for download in PDF format
Detailed PanToxiLux? P2D2 instructions are available for download in PDF format
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